Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-Blue cells | Allium cepa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
calculated from amino acid sequence | Allium cepa |
25000 | - |
SDS-PAGE | Allium cepa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Allium cepa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and S-hexylglutathione-agarose column chromatography | Allium cepa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bulb | the highest glyoxalase I concentration is found in mature bulbs | Allium cepa | - |
leaf | - |
Allium cepa | - |
root | - |
Allium cepa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.94 | - |
crude extract, at 22°C | Allium cepa |
356 | - |
after 184fold purification, at 22°C | Allium cepa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + glutathione | - |
Allium cepa | (R)-S-lactoylglutathione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glyoxalase I | - |
Allium cepa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Allium cepa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Allium cepa | calculated from amino acid sequence | - |
5.2 |
Organism | Comment | Expression |
---|---|---|
Allium cepa | glyoxlase I activity decreases in heavy metal stress (exposure to 0.5 mM CdCl2) | down |
Allium cepa | low temperature (4°C) stress shows the highest induction (2.6fold) of glyoxalase I activity, followed by salinity (exposure to 400 mM NaCl) and drought (exposure to 10% (v/v) PEG) stress for 7 days | up |
General Information | Comment | Organism |
---|---|---|
physiological function | GLOI plays an important role in the plant abiotic stress response | Allium cepa |